Tone. Don't leave for your show without it.
Tone is critical to performing stand-up comedy. It relates the attitude and very spirit of a joke or bit.
You need to consider tone in every thing you say. Tone will tell the audience if you're surprised, angry, confused, hurt, excited, or just reporting. Tone is paramount to communicating.
For emphasis, for strength, for color, inflection
Learn it, live it. Tone to the bone. Got it now. Tone.