Do you have an emotional map or gauge of your act?...ever thought of it like that? What emotion do you begin with? What emotion do you end with? What emotions do you touch on during your show?
What emotion gets to you the most? What emotions of your's seems to get to the audience the best?
Whatever the emotion or emotions you chose I'm bettin' it will be the extreme version...that's the emotion that usually translates to big laughs. Recalling not just a sad moment but the saddest moment; not just the angry moment but THE most enraged angry moment is the winner for jokes I think.